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Table 2 Fold inductions of genes identified in this study in MoMΦ or MoDC in response to LPS or Salmonella Typhimurium (STM) exposure determined by QRT-PCR

From: The response of porcine monocyte derived macrophages and dendritic cells to SalmonellaTyphimurium and lipopolysaccharide

  MoDC MoDC fold induction$ MoMΦ MoMΦ fold induction MoMΦ to MoDC
  Basal exp STM LPS Basal exp STM LPS NS STM LPS
IL1B 0.06 ± 0.04 106# ± 101 2358# ± 1268 0.11 ± 0.06 383# ± 451 1999# ± 1743 n * n
AMCF2 0.40 ± 0.20 35.3# ± 33.0 543# ± 528 0.75 ± 0.52 115# ± 107 305# ± 267 n n n
CXCL2 0.71 ± 0.28 17.4# ± 19.1 200# ± 161 0.19 ± 0.19 155# ± 102 668# ± 432 n n n
IL1A 0.28 ± 0.14 22.9# ± 25.3 229# ± 164 0.24 ± 0.12 86.5# ± 48.8 187# ± 57 n ** n
IL8 15.3 ± 13.7 5.9 ± 5.74 34.2# ± 21.6 6.78 ± 5.95 22.9# ± 13.2 113# ± 66 n n n
ISG15 0.88 ± 0.52 2.94# ± 1.22 2.00 ± 0.94 0.17 ± 0.10 22.1# ± 2.0 78.9# ± 104.4 *** n n
MARCKSL1 0.70 ± 0.60 1.82 ± 0.63 4.07# ± 2.30 0.25 ± 0.08 17.9# ± 9.1 42.9# ± 15.6 n * **
SOD2 4.28 ± 4.58 2.13 ± 0.80 12.8# ± 12.9 4.61 ± 2.55 11.3# ± 4.6 27.6# ± 11.6 n ** n
IκBα 1.15 ± 0.36 2.74# ± 1.12 8.12# ± 3.10 1.13 ± 0.57 9.64# ± 3.72 15.1# ± 4.8 n *** n
CD14 0.52 ± 0.19 3.84# ± 1.52 14.4# ± 9.6 0.35 ± 0.27 14.0# ± 9.7 18.0# ± 20.0 n n n
PPA1 1.34 ± 0.84 1.85# ± 0.7 4.83# ± 1.93 1.39 ± 0.17 4.42# ± 1.00 7.02# ± 1.73 n *** n
CD48 7.35 ± 4.25 1.83 ± 0.22 3.2# ± 1.8 9.93 ± 2.43 3.34# ± 0.71 5.33# ± 1.90 n * *
NF-κB1 1.57 ± 0.41 1.82 ± 0.61 3.49# ± 1.4 1.97 ± 0.44 2.25# ± 0.7 4.63# ± 2.20 n n n
ANXA1 15.3 ± 4.7 1.78 ± 0.9 3.74# ± 1.91 22.9 ± 13.4 1.40 ± 0.38 2.45# ± 1.01 n n n
OPN 86.1 ± 42.8 2.33 ± 1.5 3.42# ± 2.27 138 ± 72 2.12 ± 1.20 3.81# ± 2.00 n n n
PFDN2 0.09 ± 0.03 1.54 ± 0.51 2.22# ± 0.63 0.12 ± 0.04 2.12# ± 0.60 2.63# ± 0.72 n n n
PSMB4 6.18 ± 1.58 1.42 ± 0.30 1.82# ± 0.31 7.34 ± 1.39 1.73 ± 0.40 2.11# ± 0.54 n n n
MoDC only          
OLDLR1 9.73 ± 3.74 1.95# ± 0.5 3.33# ± 0.91 26.2 ± 3.0 1.43 ± 0.42 1.62 ± 0.41 *** * n
CDC42 3.52 ± 0.70 1.62# ± 0.33 1.63 ± 0.33 4.78 ± 1.43 1.60 ± 0.13 1.53 ± 0.61 n n n
MoMΦ only          
VCAM1 0.33 ± 0.25 2.32 ± 1.90 5.52 ± 4.51 0.18 ± 0.19 9.82# ± 6.24 18.9# ± 13.6 n n n
HMOX1 0.32 ± 0.20 2.32 ± 1.53 1.42 ± 0.51 0.45 ± 0.11 2.34# ± 1.11 2.44# ± 0.14 n n *
Serglycin 45.9 ± 19.9 1.52 ± 0.71 2.40 ± 1.03 35.7 ± 28.9 3.04# ± 1.32 2.84# ± 1.33 n n n
  1. $The fold inductions are presented as mean ± SD calculated based on ratios of individual treated samples relative to the mean of appropriate non-stimulated control.
  2. #The numbers signed with #represent significantly different results compared to non-stimulated (NS) cells.
  3. *Statistically significant differences between MoMΦ and MoDC with the same treatment. *p < 0.05; **p < 0.01; ***p < 0.001; n = non-significant.