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Table 2 Fold inductions of genes identified in this study in MoMΦ or MoDC in response to LPS or Salmonella Typhimurium (STM) exposure determined by QRT-PCR

From: The response of porcine monocyte derived macrophages and dendritic cells to SalmonellaTyphimurium and lipopolysaccharide

 

MoDC

MoDC fold induction$

MoMΦ

MoMΦ fold induction

MoMΦ to MoDC

 

Basal exp

STM

LPS

Basal exp

STM

LPS

NS

STM

LPS

IL1B

0.06 ± 0.04

106# ± 101

2358# ± 1268

0.11 ± 0.06

383# ± 451

1999# ± 1743

n

*

n

AMCF2

0.40 ± 0.20

35.3# ± 33.0

543# ± 528

0.75 ± 0.52

115# ± 107

305# ± 267

n

n

n

CXCL2

0.71 ± 0.28

17.4# ± 19.1

200# ± 161

0.19 ± 0.19

155# ± 102

668# ± 432

n

n

n

IL1A

0.28 ± 0.14

22.9# ± 25.3

229# ± 164

0.24 ± 0.12

86.5# ± 48.8

187# ± 57

n

**

n

IL8

15.3 ± 13.7

5.9 ± 5.74

34.2# ± 21.6

6.78 ± 5.95

22.9# ± 13.2

113# ± 66

n

n

n

ISG15

0.88 ± 0.52

2.94# ± 1.22

2.00 ± 0.94

0.17 ± 0.10

22.1# ± 2.0

78.9# ± 104.4

***

n

n

MARCKSL1

0.70 ± 0.60

1.82 ± 0.63

4.07# ± 2.30

0.25 ± 0.08

17.9# ± 9.1

42.9# ± 15.6

n

*

**

SOD2

4.28 ± 4.58

2.13 ± 0.80

12.8# ± 12.9

4.61 ± 2.55

11.3# ± 4.6

27.6# ± 11.6

n

**

n

IκBα

1.15 ± 0.36

2.74# ± 1.12

8.12# ± 3.10

1.13 ± 0.57

9.64# ± 3.72

15.1# ± 4.8

n

***

n

CD14

0.52 ± 0.19

3.84# ± 1.52

14.4# ± 9.6

0.35 ± 0.27

14.0# ± 9.7

18.0# ± 20.0

n

n

n

PPA1

1.34 ± 0.84

1.85# ± 0.7

4.83# ± 1.93

1.39 ± 0.17

4.42# ± 1.00

7.02# ± 1.73

n

***

n

CD48

7.35 ± 4.25

1.83 ± 0.22

3.2# ± 1.8

9.93 ± 2.43

3.34# ± 0.71

5.33# ± 1.90

n

*

*

NF-κB1

1.57 ± 0.41

1.82 ± 0.61

3.49# ± 1.4

1.97 ± 0.44

2.25# ± 0.7

4.63# ± 2.20

n

n

n

ANXA1

15.3 ± 4.7

1.78 ± 0.9

3.74# ± 1.91

22.9 ± 13.4

1.40 ± 0.38

2.45# ± 1.01

n

n

n

OPN

86.1 ± 42.8

2.33 ± 1.5

3.42# ± 2.27

138 ± 72

2.12 ± 1.20

3.81# ± 2.00

n

n

n

PFDN2

0.09 ± 0.03

1.54 ± 0.51

2.22# ± 0.63

0.12 ± 0.04

2.12# ± 0.60

2.63# ± 0.72

n

n

n

PSMB4

6.18 ± 1.58

1.42 ± 0.30

1.82# ± 0.31

7.34 ± 1.39

1.73 ± 0.40

2.11# ± 0.54

n

n

n

MoDC only

         

OLDLR1

9.73 ± 3.74

1.95# ± 0.5

3.33# ± 0.91

26.2 ± 3.0

1.43 ± 0.42

1.62 ± 0.41

***

*

n

CDC42

3.52 ± 0.70

1.62# ± 0.33

1.63 ± 0.33

4.78 ± 1.43

1.60 ± 0.13

1.53 ± 0.61

n

n

n

MoMΦ only

         

VCAM1

0.33 ± 0.25

2.32 ± 1.90

5.52 ± 4.51

0.18 ± 0.19

9.82# ± 6.24

18.9# ± 13.6

n

n

n

HMOX1

0.32 ± 0.20

2.32 ± 1.53

1.42 ± 0.51

0.45 ± 0.11

2.34# ± 1.11

2.44# ± 0.14

n

n

*

Serglycin

45.9 ± 19.9

1.52 ± 0.71

2.40 ± 1.03

35.7 ± 28.9

3.04# ± 1.32

2.84# ± 1.33

n

n

n

  1. $The fold inductions are presented as mean ± SD calculated based on ratios of individual treated samples relative to the mean of appropriate non-stimulated control.
  2. #The numbers signed with #represent significantly different results compared to non-stimulated (NS) cells.
  3. *Statistically significant differences between MoMΦ and MoDC with the same treatment. *p < 0.05; **p < 0.01; ***p < 0.001; n = non-significant.