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Table 1 Procedures applied to embryos on breakout according to incubation period

From: Influence of temperature and humidity manipulation on chicken embryonic development

Procedure Incubation day
0-2 3-15 15-20
Weighing x x x
Candling x x x
Detection of embryo   x x
Assessment of HR and VMM   x x
Embryo euthanasia    
  (<4°C, 4 h) x x  
  (100%, CO2)    x
Detection and measurement of blastodisc/blastoderm x   
Detection and measurement of embryo   x x
Weighing of embryo   x x
Weighing of the albumen, yolk and shell x x x