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Figure 3 | BMC Veterinary Research

Figure 3

From: Mycoplasma synoviaeenolase is a plasminogen/fibronectin binding protein

Figure 3

Determination of the localization of M. synoviae enolase. (A) Western blot analysis. Lane 1: Total cellular proteins of M. synoviae. Lane 2: Purified rMsEno was used as a positive control. Lane 3: The cytosolic proteins of M. synoviae. Lane 4: The membrane proteins of M. synoviae. Lane 5: BSA was used as a negative control. (B) Immunoelectron microscopy examination. Arrows pointed the M. synoviae enolase to be stained with goat anti-rabbit IgG labeled with 12 nm diametral colloidal gold particles. (C) Non-immunized rabbit serum showed no any binding activity.

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