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Figure 1 | BMC Veterinary Research

Figure 1

From: The presence of six potentially pathogenic viruses in pigs suffering from post-weaning multisystemic wasting syndrome

Figure 1

Prevalence of viruses in individual groups of pigs. Viruses were detected in pooled tissue samples (lymph nodes, liver and spleen) of diseased pigs of different age and lymph nodes of healthy animals. Total DNA or RNA was isolated by Chelex resins or TRIzol reagent, respectively. RNA was transcribed into cDNA using random hexamers (PTV) or gene-specific primer (PRRSV) and SuperScript III reverse transcriptase. Viral genomes were detected by PCR, RT-PCR or real-time PCR based on SYBR Green (TTSuV1 and TTSuV2).

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