Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Table 4 Primers and probes used in cloning, CDV RT-iiPCR and real-time RT-PCR

Primer	Nucleotide sequence (5'-3')	Note*
CDV-F	AGCTAGTTTCATCTTAACTATCAAATT	CDV real-time RT-PCR forward primer^a
CDV-R	TTAACTCTCCAGAAAACTCATGC	CDV real-time RT-PCR reverse primer^a
CDV-P	FAM-ACCCAAGAGCCGGATACATAGTTTCAATGC-BHQ1	CDV real-time RT-PCR probe^a
CDV-F1	ATGGCTAGCCTTCTTAAAAGCCTCACA	CDV cloning primer (nt108-134)*
CDV-R1	TTCCGATCATCGTCATTTCCATCA	CDV cloning primer (nt1570-1547)*
CDViiF	CCGGAAATCAACGGACCTAAATT	CDV RT-iiPCR primer (nt291 ~ 313)*
CDViiR	GTCGTCTATGATCCTCTGGATCAA	CDV RT-iiPCR primer (nt389 ~ 366)*
CDViiP	FAM-CAGTATCCTCTCCTTGTTCGTGGA-MGB-NFQ	CDV RT-iiPCR probe (nt329 ~ 352)*

*Nucleotide position is based on GenBank accession no. AF305419.1; ^aElia et al. [42].

ISSN: 1746-6148