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Table 1 Comparison of analytical sensitivity between validated CDV real-time RT-PCR and RT-iiPCR using viral RNA of Onderstepoort strain

From: Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Sample dilution

Real-time RT-PCR

RT-iiPCR No. positive/total

No. positive/total

Ct

NTC*

-

 

-

Positive control

+

 

+

10-1

2/2

34.09

3/3

10-2

2/2

36.99

3/3

10-3

1/2

40.2

2/3

10-4

0/2

 

0/3

10-5

0/2

 

0/3

10-6

0/2

 

0/3

  1. *NTC-No template control.
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BMC Veterinary Research

ISSN: 1746-6148

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