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Table 1 Comparison of analytical sensitivity between validated CDV real-time RT-PCR and RT-iiPCR using viral RNA of Onderstepoort strain

From: Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Sample dilution Real-time RT-PCR RT-iiPCR No. positive/total
No. positive/total Ct
NTC* -   -
Positive control +   +
10-1 2/2 34.09 3/3
10-2 2/2 36.99 3/3
10-3 1/2 40.2 2/3
10-4 0/2   0/3
10-5 0/2   0/3
10-6 0/2   0/3
  1. *NTC-No template control.