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Table 2 Primer sequences used for qualitative and quantitative (q) real time RT-PCR, and RACE and the expected product size

From: Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene

Primer name

Assay

Sequence (5′-3′)

Exon position

Target gene

Amplicon sizes (bp)

GHRL-1 F

RT-PCR

TTTCTGAGCCCTGAACATCAG

1

GHRL

305 canonical (wild-type) preproghrelin

GHRL-4R

 

GAGAACAGACAGGTGGTTGG

4

  

GHRL-5OF

RACE

ATGAATGAACACTGCGTTTGCT

N/A

GHRL

174

GHRL-5OR

 

CAGTTTCTGATGTTCAGGGCTC

1

GHRL-5IF

RACE

GAACACTGCGTTTGCTGGCT

N/A

GHRL

130

GHRL-5IR

 

GGCCAAGTCCATCCAGAGCA

1

GHRL-2F

qRT-PCR

CTAAGAAGCCGTCAGGCAGACT

2

GHRL (preproghrelin)

153

GHRL-2R

 

GGGACTGAGCCCCTGACA

3

GHRL1_3F

qRT-PCR

CAGAAACTGCAGTTCAATGC

1 / 3

GHRL (Δex2 preproghrelin)

224

GHRL-4R

 

CACGTGGTCTCGGAAGTGT

4

GHRL1_4F

qRT-PCR

CTGCAGAAACCCTGGCTGA

1 / 4

GHRL (Δex2,3 preproghrelin)

109

GHRL-4R

 

CACGTGGTCTCGGAAGTGT

4

GOAT-mF

qRT-PCR

GGTTTCAAGCTCGAGTTGAAGG

N/A

MBOAT4 (GOAT)

212

GOAT-mR

 

AGTAGGTGAGTTTGAAGAGCCC

GAPDH-F

qRT-PCR

CCTGGAGAAACCTGCCAAGT

N/A

GAPDH

209

GAPDH-R

 

GCCAAATTCATTGTCGTACCA