From: Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene
Primer name | Assay | Sequence (5′-3′) | Exon position | Target gene | Amplicon sizes (bp) |
---|---|---|---|---|---|
GHRL-1 F | RT-PCR | TTTCTGAGCCCTGAACATCAG | 1 | GHRL | 305 canonical (wild-type) preproghrelin |
GHRL-4R | Â | GAGAACAGACAGGTGGTTGG | 4 | Â | Â |
GHRL-5OF | RACE | ATGAATGAACACTGCGTTTGCT | N/A | GHRL | 174 |
GHRL-5OR | Â | CAGTTTCTGATGTTCAGGGCTC | 1 | ||
GHRL-5IF | RACE | GAACACTGCGTTTGCTGGCT | N/A | GHRL | 130 |
GHRL-5IR | Â | GGCCAAGTCCATCCAGAGCA | 1 | ||
GHRL-2F | qRT-PCR | CTAAGAAGCCGTCAGGCAGACT | 2 | GHRL (preproghrelin) | 153 |
GHRL-2R | Â | GGGACTGAGCCCCTGACA | 3 | ||
GHRL1_3F | qRT-PCR | CAGAAACTGCAGTTCAATGC | 1 / 3 | GHRL (Δex2 preproghrelin) | 224 |
GHRL-4R | Â | CACGTGGTCTCGGAAGTGT | 4 | ||
GHRL1_4F | qRT-PCR | CTGCAGAAACCCTGGCTGA | 1 / 4 | GHRL (Δex2,3 preproghrelin) | 109 |
GHRL-4R | Â | CACGTGGTCTCGGAAGTGT | 4 | ||
GOAT-mF | qRT-PCR | GGTTTCAAGCTCGAGTTGAAGG | N/A | MBOAT4 (GOAT) | 212 |
GOAT-mR | Â | AGTAGGTGAGTTTGAAGAGCCC | |||
GAPDH-F | qRT-PCR | CCTGGAGAAACCTGCCAAGT | N/A | GAPDH | 209 |
GAPDH-R | Â | GCCAAATTCATTGTCGTACCA |