Effect of Agrimonia procera extract (APE) on cytokine mRNA expression by porcine PBMC. Relative mRNA concentrations of TNFα (A), IL-1β (B) and IL-10 (C) were analyzed in non-stimulated and LPS (1 μg/ml)-stimulated PBMC (2 × 106 per ml RPMI 1640 medium) treated with 0, 0.05, 0.1 or 0.2% APE for 20 h. PBMC were isolated from 6 healthy piglets. The relative mRNA expression was normalized to that of RPP0 and SDHA. Data represent mean ± SD (n = 6). a,bBars with different superscript letters differ significantly (P < 0.05, Fisher's test).