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Figure 4 | BMC Veterinary Research

Figure 4

From: Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation

Figure 4

Isoelectric focussing of alkaline phosphatase in bovine tissue and nasal secretion. Bovine tissue extracts and nasal secretion separated on isoelectric focusing gels over a pH 3-7 range and stained with 5-Bromo-4-chloro-3-indolyl phosphate with nitro blue tetrazolium chloride (BCIP/NBT) substrate. M = IEF standard protein marker (stained with Coomassie blue); -ve = cathode; +ve = anode. Two different samples of NS were run in duplicate. Samples of extracts from nasal mucosa, bone and intestine were run in duplicate with liver and kidney extract being run in single tracks. Annotated boxes around isoforms bands as discussed in the text.

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