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Figure 2 | BMC Veterinary Research

Figure 2

From: The different molecular forms of urine neutrophil gelatinase-associated lipocalin present in dogs with urinary diseases

Figure 2

Preparation of recombinant MMP-9 protein for mouse immunization. (A) Partial MMP-9 coding region (nucleotide1580-2131) was amplified by RT-PCR using cDNA (lane +) generated from RNA of canine testis. (B) The recombinant MMP-9 with expected size of 22 kilo-Dalton (kDa) was expressed in E. coli and purified by metal affinity chromatography (lane 1).

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