Figure 2From: The different molecular forms of urine neutrophil gelatinase-associated lipocalin present in dogs with urinary diseasesPreparation of recombinant MMP-9 protein for mouse immunization. (A) Partial MMP-9 coding region (nucleotide1580-2131) was amplified by RT-PCR using cDNA (lane +) generated from RNA of canine testis. (B) The recombinant MMP-9 with expected size of 22 kilo-Dalton (kDa) was expressed in E. coli and purified by metal affinity chromatography (lane 1).Back to article page