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Figure 3 | BMC Veterinary Research

Figure 3

From: Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approach

Figure 3

Agarose gel electrophoresis of35S-PGs from normal articular cartilage (Initial) and healing tissue (Final, 180 days) formed either in absence (No treatment) or in presence of chitosan-GP. Normal articular cartilage samples collected during the first arthroscopy to produce the osteochondral defect (~100 mg, wet weight), as well as samples of the healing tissues formed in presence or absence of chitosan-GP, were metabolically labeled with 35S-sulfate. 35S-PGs were isolated from the conditioned media (M) and the tissue explants (T). These compounds were analyzed by agarose gel electrophoresis (PDA buffer), and localized in the gel by Toluidine Blue staining (left) and radioautography (right).

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