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Table 1 rtPCR results obtained from the enrichment protocols evaluated in experiment 1 for Y. pseudotuberculosis and Y. enterocolitica

From: Evaluation of different enrichment methods for pathogenic Yersiniaspecies detection by real time PCR

Enrichment protocol Y. enterocoliticartPCR Y. pseudotuberculosisrtPCR
Pos (Ct ≤) Pos (Ct >) Neg. Pos (Ct ≤) Pos (Ct >) Neg.
CASO 5 2 1 4 0 0
PSB 3 5 0 0 4 0
ITC 5 2 1 0 3 1
PBS1 5 2 1 0 4 0
PBS2 7 1 0 4 0 0
PBS3 6 1 1 4 0 0
PBS4 5 3 0 4 0 0
PBSMSB 5 3 0 4 0 0
PBSSSB 6 1 1 3 1 0
  1. Pos (Ct ≤): rtPCR positive result after enrichment with Ct value minor or similar (±0.5) than Ct values from direct rtPCR.
  2. Pos (Ct >): rtPCR positive result after enrichment with Ct value major than Ct values from direct rtPCR.
  3. Neg: rtPCR negative result.