Skip to main content

Table 1 rtPCR results obtained from the enrichment protocols evaluated in experiment 1 for Y. pseudotuberculosis and Y. enterocolitica

From: Evaluation of different enrichment methods for pathogenic Yersiniaspecies detection by real time PCR

Enrichment protocol

Y. enterocoliticartPCR

Y. pseudotuberculosisrtPCR

Pos (Ct ≤)

Pos (Ct >)

Neg.

Pos (Ct ≤)

Pos (Ct >)

Neg.

CASO

5

2

1

4

0

0

PSB

3

5

0

0

4

0

ITC

5

2

1

0

3

1

PBS1

5

2

1

0

4

0

PBS2

7

1

0

4

0

0

PBS3

6

1

1

4

0

0

PBS4

5

3

0

4

0

0

PBSMSB

5

3

0

4

0

0

PBSSSB

6

1

1

3

1

0

  1. Pos (Ct ≤): rtPCR positive result after enrichment with Ct value minor or similar (±0.5) than Ct values from direct rtPCR.
  2. Pos (Ct >): rtPCR positive result after enrichment with Ct value major than Ct values from direct rtPCR.
  3. Neg: rtPCR negative result.