Table 1 Genes and nucleotide sequences of the primers used in qRT-PCR
Gene reference | Primers (5’ to 3’ nucleotide sequences) | Annealing temperature°C | Product size pb |
|---|---|---|---|
GAPDH [12] | Forward - CCATCTTCCAGGAGCGAGGAT | 60 | 97 |
Reverse - TTCTCCATGGTGGTGAAGAC | |||
Osteocalcin [5] | Forward - GAGGGCAGCGAGGTGGTGAG | 62 | 134 |
Reverse - TCAGCCAGCTCGTCACAGTTGG | |||
Bone sialoprotein (BSP) [12] | Forward - TTGCTCAGCATTTTGGGAAT | 60 | 295 |
Reverse - AACGTGGCCGATACTTAAAGAC | |||
Osterix [5] | Forward - ACGACACTGGGCAAAGCAG | 60 | 285 |
Reverse - CATGTCCAGGGAGGTGTAGAC |