Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis

Mercier, Elise; Peters, Iain R; Farnir, Frédéric; Lavoué, Rachel; Day, Michael; Clercx, Cécile; Peeters, Dominique

doi:10.1186/s12917-014-0187-6

BMC Veterinary Research

Table 9 Allele-specific primers and positive control used for genotyping

From: Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis

SNP	Primer	Primer sequence (5′-3′)	Positive control
*TLR2*
C137A	Forward	CCGCTCCAGATCTTTGAAgTC	CCGCTCCAGATCTTTGAACTCCATGCATTGGCAACAGTGACCTTCGGGATT
(110 bp) [60°C]	Forward	CCGCTCCAGATCTTTGAAgTA	CCGCTCCAGATCTTTGAACTACATGCATTGGCAACAGTGACCTTCGGGATT
(110 bp) [60°C]	Reverse	AATCCCGAAGGTCACTGTTG
C1547T	Forward	GCCTCCTTCTTACCCACCTT
(81 bp) [60°C]	Reverse	GGAATCCAGTTGCTCCTaCG	GGAATCCAGTTGCTCCTTCGAGAAAGAATTTGTAAGGTGGGTAAGAAGGAGGC
(81 bp) [60°C]	Reverse	GGAATCCAGTTGCTCCTaCA	GGAATCCAGTTGCTCCTTCAAGAAAGAATTTGTAAGGTGGGTAAGAAGGAGGC
*TLR4*
C107T	Forward	GGAAAGGAGAGAGGGCAGTT
(101 bp) [60°C]	Reverse	AGCTCCATGCATTGGTAAGaAG	AGCTCCATGCATTGGTAAGTAGGCATGCACCTCAACTGCCCTCTCTCCTTTCC
(101 bp) [60°C]	Reverse	AGCTCCATGCATTGGTAAGaAA	AGCTCCATGCATTGGTAAGTAAGCATGCACCTCAACTGCCCTCTCTCCTTTCC
T500C	Forward	CAATCTTATCCATTCCTTCAAcCT	CAATCTTATCCATTCCTTCAAGCTTGCGTATTTCTCTAACATGCCCAACCTGGAG
(58 bp) [60°C]	Forward	CAATCTTATCCATTCCTTCAAcCC	CAATCTTATCCATTCCTTCAAGCCTGCGTATTTCTCTAACATGCCCAACCTGGAG
(58 bp) [60°C]	Reverse	CTCCAGGTTGGGCATGTTAG
A600C	Forward	CTAACATGCCCAACCTGGAG
(110 bp) [60°C]	Reverse	TAAAGAAAGGTTGAGTAGTGGCtTT	TAAAGAAAGGTTGAGTAGTGGCATTTGATGTAGACGTTCTCCAGGTTGGGCATGTTAG
(110 bp) [60°C]	Reverse	TAAAGAAAGGTTGAGTAGTGGCtTG	TAAAGAAAGGTTGAGTAGTGGCATGTGATGTAGACGTTCTCCAGGTTGGGCATGTTAG
A688G	Forward	GGTTCCTTTAAAGAAATTAAACTCgATA	GGTTCCTTTAAAGAAATTAAACTCCATAAACTGACTTTGAGAAGCCAGCCAGACCTTGAATA
(97 bp) [60°C]	Forward	GGTTCCTTTAAAGAAATTAAACTCgATG	GGTTCCTTTAAAGAAATTAAACTCCATGAACTGACTTTGAGAAGCCAGCCAGACCTTGAATA
(97 bp) [60°C]	Reverse	AGCCAGCCAGACCTTGAATA
G1039A	Forward	ATGGCAACGGTTGGAAATAG
(66 bp) [60°C]	Reverse	AGAGAGTCCAGCTCCCAaGC	AGAGAGTCCAGCTCCCATGCCTAAGTTACAATTAACTATTTCCAACCGTTGCCAT
(66 bp) [60°C]	Reverse	AGAGAGTCCAGCTCCCAaGT	AGAGAGTCCAGCTCCCATGTCTAAGTTACAATTAACTATTTCCAACCGTTGCCAT
A1571T	Forward	ATGGCTGATAATTCCTTTCC
(163 bp) [55°C]	Reverse	GGCTATTGTGACTCATATTTAaCA	GGCTATTGTGACTCATATTTATCAAGGGAGTTGTCCGGAAAGGAATTATCAGCCAT
(163 bp) [55°C]	Reverse	GGCTATTGTGACTCATATTTtTCT	GGCTATTGTGACTCATATTTATCTAGGGAGTTGTCCGGAAAGGAATTATCAGCCAT
G1807A	Forward	TGGGTCAAAGACCACAGACA
(53 bp) [60°C]	Reverse	GCACACACCATTTGTTCAAgTTC	GCACACACCATTTGTTCAACTTCCACCAAGAGCTGTCTGTGGTCTTTGACCCA
(53 bp) [60°C]	Reverse	GCACACACCATTTGTTCAAgTTT	GCACACACCATTTGTTCAACTTTCACCAAGAGCTGTCTGTGGTCTTTGACCCA
*TLR9*
G1138A	Forward	GCGCAGACTCAACCTGTCCTT
(152 bp) [55°C]	Reverse	GCGACTGGAGCGTGGTgTC	GCGACTGGAGCGTGGTCTCCTTATGATAATTGAAGGACAGGTTGAGTCTGCGC
(152 bp) [55°C]	Reverse	GCGACTGGAGCGTGGTgTT	GCGACTGGAGCGTGGTCTTCTTATGATAATTGAAGGACAGGTTGAGTCTGCGC
A1372C	Forward	GCGGCTGCCACAGGGGAGGT
(95 bp) [60°C]	Reverse	GAGCCGGGGGTGCCCAGaGT	GAGCCGGGGGTGCCCAGTGTCACAGTCTGCCTCTACCTCCCCTGTGGCAGCCGC
(95 bp) [60°C]	Reverse	AGCCGGGGGTGCCCAGaGG	GAGCCGGGGGTGCCCAGTGGCACAGTCTGCCTCTACCTCCCCTGTGGCAGCCGC
A2158G	Forward	CTGGACCTCAGCGGCAACAG
(58 bp) [60°C]	Reverse	CGGCCAGGGCAAAAAAcCT	CGGCCAGGGCAAAAAAGCTCACGAAGCCGATGCTGTTGCCGCTGAGGTCCAG
(58 bp) [60°C]	Reverse	CGGCCAGGGCAAAAAAcCC	CGGCCAGGGCAAAAAAGCCCACGAAGCCGATGCTGTTGCCGCTGAGGTCCAG
G2927A	Forward	CTCCTGCGTGCCAGCTTCCT
(113 bp) [55°C]	Reverse	AGCCGCACATAGCGGGtGC	AGCCGCACATAGCGGGAGCCTGTTGGGCCAGCAGGAAGCTGGCACGCAGGAG
(113 bp) [55°C]	Reverse	AGCCGCACATAGCGGGtGT	AGCCGCACATAGCGGGAGTCTGTTGGGCCAGCAGGAAGCTGGCACGCAGGAG

The polymorphic base is in bold, the additional inserted 3′ mismatch is in lowercase, the amplicon size in base pairs (bp) and annealing temperature [°C] used for the qPCT are shown below the SNP name.

Back to article page

ISSN: 1746-6148

Contact us

General enquiries: journalsubmissions@springernature.com