Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

Table 1 Antibodies and details of the protocols applied for the immunohistochemical examination

Markers	Antibodies(mouse anti-human) clone and producer	Dilution*	Unmasking	Incubation time**
Cytokeratin 5/6	Clone D5/16 B4	1:100	CCR	24 min
Cytokeratin 5/6	Dakocytomation	1:100	+ protease 2 min	24 min
Cytokeratin 14	NCL –L-LL002	1:20	CCR	18 min
Cytokeratin 14	Novocastra	1:20	CCR	18 min
Cytokeratin 8/18	NCL-L-5D3	1:20	protease 8 min	24 min
Cytokeratin 8/18	Dakocytomation	1:20	protease 8 min	24 min
Pancytokeratin	Clone AE1/AE3	1:100	CCR	16 min
Pancytokeratin	Dakocytomation	1:100	CCR	16 min
Calponin	Clone Calp	1:200	CCR	12 min
Calponin	Dakocytomation	1:200	CCR	12 min
Vimentin	Clone V9	1:100	CCR	18 min
Vimentin	Dakocytomation	1:100	CCR	18 min
α-smooth muscle actin	Clone 1A4	1:100	no	10 min
α-smooth muscle actin	Dakocytomation	1:100	no	10 min
Estrogen receptor	NCL-ER-6 F11	1:40	CCS	14 min
Estrogen receptor	Novocastra	1:40	CCS	14 min
Progesteron receptor	Clone: PR10A9	1:100	CCE	18 min
Progesteron receptor	Immunotech	1:100	CCE	18 min

CCR, cell conditioning reduced: 30 minutes at 95°C; CCS, cell conditioning standard: 60 minutes at 95°C; CCE, cell conditioning extended: 90 minutes at 95°C.
*The primary antibody was diluted in the Antibody Diluent (Ventana Medical System Inc., Tucson, AZ).
**Incubation time for primary antibody; min, minutes.

ISSN: 1746-6148