Table 1 Analytical methods used in ADVIA®1650 and ADVIA®1800 Chemistry Systems for blood analysis from brown bears
Analytes | Analytical concepts |
|---|---|
ALT | Modified IFCC |
Amylase | Ethylidene blocked-pNPG7 |
AP | Modified IFCC |
AST | Modified IFCC |
β-HBA | Enzymatic/NAD + −NADH |
Total bilirubin | Vanadate oxidation |
Inorganic phosphorus | Phosphomolybdate/UV |
FFA | Enzymatic-colorimetric |
Bile acids | Enzymatic amplification/Thio-NAD-Thio-NADH |
GGT | Modified IFCC |
Glucose | Hexokinase |
GD | DGKC/NADH-NAD+ |
Iron | Ferrozine |
Calcium | CPC |
Potassium | Ion selective electrode (ISE), diluted |
Chloride | Ion selective electrode (ISE), diluted |
Cholesterol | Enzymatic/trinder |
CK | NAC activated (DGKC) |
Creatinine | Jaffe, kinetic |
LD | Enzymatio (L-P) / TRIS / NAD + −NADH |
Lipase | Colorimetric rate |
Magnesium | Xylidyl blue |
Sodium | Ion selective electrode (ISE), diluted |
Total Protein | Biuret |
Triglycerides | Enzymatic/trinder without serum blank |
Urea | Enzymatic: urease with GLDH |
Uric Acid | Enzymatic/trinder |