Nucleotide sequence of NCL-affected Chinese Crested around
. (A) Shows the NextGENe Viewer display of the alignment of WGS reads around MFSD8:c.843delT. All 30 reads that span the 3 consecutive Ts that make up codon 282 show a highlighted 1 bp deletion. The read that is the third from the top starts within the TTT sequence and the read at the bottom ends within the TTT sequence. Because these 2 reads aligned with a T at the position corresponding to MFSD8:c.843, the NextGENe software scored the variant at C.843 as a heterozygous T deletion. (B) Automated Sanger sequencing indicated that the NCL-affected Chinese Crested had a homozygous T deletion.