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Table 3 Experiment design for grouping, sampling, and analysis

From: Supplementation of dietary germanium biotite enhances induction of the immune responses by foot-and-mouth disease vaccine in cattle

Farms Number of cattle Samples Analysis of
Control Germanium biotite
A 26 22 Serum, saliva, feces Inhibition percentage, ELISA for antibody levels (IgG and IgA), lysozyme activity
B 13 18 Serum, saliva, feces Inhibition percentage, ELISA for antibody levels (IgG and IgA), lysozyme activity, virus neutralization test
C 5 5 Serum, saliva, feces, PBMC Inhibition percentage, ELISA for antibody levels (IgG and IgA), lysozyme activity, PBMC (phenotype, cytokines, proliferation)
  1. Among 89 cattle at 6-8 months of age, 45 cattle were raised with feeds supplemented with 0.5% germanium biotite from 1 week before vaccination to the end of experiment (germanium biotite group) and the rest of them were raised without supplementation (control group).