Inhibition of nestin expression by VEGF receptor antagonist. A. qPCR analysis of VEGF expression in hMSCs cultured at 21% or 1% O2 for 24 hrs. Bars represent mean VEGF expression normalized to ribosomal protein L13 ± SEM expression relative to 21% O2 (n = 3). B. qPCR analysis of nestin expression after 24 hrs of culture at 21% and 1% O2 in the presence or absence of 10 μM of CBO-P11, a VEGF receptor antagonist. Treatment with CBO-P11 prevents the increase in Nestin observed in hypoxic conditions. Bars represent mean nestin expression normalized to ribosomal protein L13 ± SEM expression relative to 21% O2 control (n = 3). *Indicates p < 0.05.