Alkaline phosphatase activity, calcium deposition and nestin expression during osteogenic differentiation of canine MSCs. A. Representative wells demonstrating the effects of time in osteogenic media on alkaline phosphatase activity (ALP) and calcium deposition (Alizarin red staining; AR). B. Effects of time in osteogenic media on Nestin expression in cMSCs. Bars represent mean nestin expression normalized to β2-microglobulin ± SEM relative to day 0 (n = 5).