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Figure 4 | BMC Veterinary Research

Figure 4

From: Recombinant porcine epidermal growth factor-secreting Lactococcus lactispromotes the growth performance of early-weaned piglets

Figure 4

In vitro biological activity of porcine EGF produced by L. lactis . Twenty-four hours after serum deprivation, L929 mouse fibroblast cells were cultured for an additional 24 h in 2.0 ml of DMEM containing 20 ng/ml of rhEGF, 20 μl of supernatant from LL-EV, and 10 μl or 20 μl of supernatant from LL-pEGF. Results are expressed as the cell numbers of each treatment and represent the means of three independent experiments performed in triplicate. Different lowercase letters denote statistical differences between groups at P < 0.05. rhEGF, recombinant porcine epidermal growth factor; LL-EV, empty vector-expressing Lactococcus lactis; LL-pEGF, pEGF-secreting Lactococcus lactis.

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