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Table 1 Effects of dietary treatment (T, n = 18) and time period (P, n = 18) on DMI, ruminal pH, blood count, blood gas, haematological profile and acute phase proteins

From: Effect of induced ruminal acidosis on blood variables in heifers

  Treatment1 Period2 P-value SEM
Trait CT MS HS 1 2 3 T P T x P  
DMI, kg/d 7.7a 6.9a 5.1b 6.2 6.6 6.9 0.002 0.426 0.571 0.39
Nadir ruminal pH 5.69a 5.47ab 5.44b 5.23b 5.75a 5.62a 0.042 0.003 0.307 0.073
Mean ruminal pH 6.50a 6.34b 6.31b 6.15b 6.48a 6.52a 0.012 0.001 0.116 0.076
Max ruminal pH 7.13 7.09 7.08 6.97b 7.08ab 7.25a 0.423 0.001 0.054 0.090
Blood count and gas
HGB, g/dL 11.1ab 10.9b 11.4a 11.7a 10.8b 10.9b 0.010 0.001 0.526 0.23
HCT, % 33.8ab 32.8b 34.1a 35.4a 32.3b 33.0b 0.027 <0.001 0.563 0.60
PLT, K/μL 506b 481b 601a 493 564 530 0.008 0.177 0.043 78.4
MPV, fl 4.2 4.1 3.9 4.0 4.2 4.0 0.542 0.840 0.266 0.26
pCO2, mmHg 52.0 50.3 50.4 50.4 50.9 51.4 0.126 0.502 0.137 0.63
pO2, mmHg 61.7 72.3 71.1 42.1b 71.4ab 91.6a 0.450 0.003 0.454 7.10
HCO3-, mmol/L 31.8α 31.3αβ 30.6β 31.8 31.3 30.7 0.071 0.127 0.081 0.45
O2Hb, % 87.6 87.9 86.3 77.5b 89.9a 94.5a 0.728 <0.001 0.683 2.01
RHb, % 9.9 9.8 12.4 20.4a 8.6b 3.1b 0.381 <0.001 0.721 1.96
sO2m, % 89.9 90.3 87.5 79.2b 91.4a 97.0a 0.393 <0.001 0.715 2.12
Haematological profile and acute phase proteins
Glucose, mmol/L 4.34 4.37 4.32 4.42α 4.26β 4.35αβ 0.686 0.098 0.891 0.104
CHOL, mmol/L 3.52 3.37 3.45 3.27b 3.28b 3.79a 0.446 0.005 0.548 0.130
NEFA, meq/L 0.23 0.20 0.27 0.24 0.21 0.25 0.155 0.624 0.555 0.025
β-HB, mmol/L 0.28 0.31 0.29 0.26 β 0.30αβ 0.31 α 0.440 0.069 0.406 0.016
AST, U/L 78.2ab 72.1b 82.0a 76.1αβ 74.4β 81.7α 0.007 0.053 0.092 1.70
γGT, U/L 19.2 19.4 18.6 17.7b 19.2ab 20.4a 0.527 0.031 0.622 1.33
LBP, μg/ml 5.9b 9.5a 10.5a 10.5a 7.4b 7.9b <0.001 0.014 0.221 0.92
  1. 1CT = control; MS = medium starch; HS = high starch.
  2. 2 Experimental periods.
  3. a–b Means within a row with different superscripts differ (P < 0.05). α– β Means within a row with different superscripts differ (P < 0.10).
  4. HGB = haemoglobin; HCT = haematocrit; PLT = platelet count; MPV = mean platelet volume; pCO2 = partial pressure of carbon dioxide; pO2 = partial pressure of oxygen; HCO3 = bicarbonate level; O2Hb = oxyhaemoglobin; RHb = reduced haemoglobin; sO2m = measured oxygen saturation; CHOL = cholesterol; β-HB = β-hydroxybutyrate; AST = aspartate aminotransferase; γGT = γ-glutamyl transferase; LBP = lipopolysaccharide-binding protein.
  5. The day (D) effect (P) was as follows: DMI (< 0.001), mean pH (< 0.001), max pH (< 0.001), HGB (0.017), total protein (0.015), glucose (< 0.001), CHOL (0.014), NEFA (< 0.001), β-HB (0.012), AST (0.054) and LBP (0.012). The P value was > 0.05 for the other variables tested.
  6. The interaction P x D effect (P) was as follows: DMI (0.011), mean pH (0.008), nadir pH (0.015), HGB (0.041), pO2 (0.049), glucose (0.001) and NEFA (0.026). The P value was > 0.05 for the other variables tested.
  7. The interaction T x P x D effect (P) was as follows: DMI (0.008) and mean pH (0.047). The P value was > 0.05 for the other variables tested.