Group | Immunizationa |
n
| Mortality (%) | Gross lesionb |
---|
Liver | Spleen |
---|
Prime | Booster | ENc | NFd | EN | NF |
---|
A | JOL916/JOL1229 | JOL916/JOL1229 | 10 | 10* | 0.7±1.1* | 0.4±1.0* | 0.4±1.0* | 0.7±0.9* |
B | JOL916/JOL1229 | JOL916 | 10 | 10* | 0.6±1.0* | 0.8±1.1* | 0.7±1.2* | 0.5±1.0** |
C | PBS | PBS | 10 | 70 | 2.1±1.4 | 2.1±1.4 | 2.1±1.4 | 2.3±1.3 |
- a JOL916 is a vaccine strain and JOL1229 is an LTB protein-secreting strain for adjuvant. JOL916/JOL1229 is a mixture consisting of 4 parts JOL916 and 1 part JOL1229. The dose of immunization was 1 × 108 CFU in a volume of 200 μl.
- b Gross lesion score (mean ± SEM).
- c Enlargement.
- d Necrotic foci.
- Asterisks indicate a significant difference compared to the control group (*p < 0.05, **p < 0.01). n, numbers of the chickens used.