Oral immunization with an attenuated Salmonella Gallinarum mutant as a fowl typhoid vaccine with a live adjuvant strain secreting the B subunit of Escherichia coliheat-labile enterotoxin

Table 1 Immunization, mortality, and gross lesions in the internal organs of chickens after challenge in experiment 1

Group	Immunization^a		n	Mortality (%)	Gross lesion^b
	Immunization^a				Liver		Spleen
	Prime	Booster			EN^c	NF^d	EN	NF
A	JOL916/JOL1229	JOL916/JOL1229	10	10^*	0.7±1.1^*	0.4±1.0^*	0.4±1.0^*	0.7±0.9^*
B	JOL916/JOL1229	JOL916	10	10^*	0.6±1.0^*	0.8±1.1^*	0.7±1.2^*	0.5±1.0^**
C	PBS	PBS	10	70	2.1±1.4	2.1±1.4	2.1±1.4	2.3±1.3

^a JOL916 is a vaccine strain and JOL1229 is an LTB protein-secreting strain for adjuvant. JOL916/JOL1229 is a mixture consisting of 4 parts JOL916 and 1 part JOL1229. The dose of immunization was 1 × 10⁸ CFU in a volume of 200 μl.
^b Gross lesion score (mean ± SEM).
^c Enlargement.
^d Necrotic foci.
Asterisks indicate a significant difference compared to the control group (^*p < 0.05, ^**p < 0.01). n, numbers of the chickens used.

ISSN: 1746-6148