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Table 1 Outline of examined BLV-infected but clinically normal cattle

From: Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR

Cattle Age (years) WBC (/μl) Lymphocyte (/μl) (%) Clinical stagea ELISAb Proviral loadc
(Copies/1 × 105cells)
Syncytium assayd
(Number/5 × 106cells)
BLV-free normal cattle
N790 2.5 9,540 6,382 (66.9) - - 0 0
N791 2.5 8,260 4,956 (60.0) - - 0 0
BLV-infected cattle
1) Cattle with < 100 proviral load
N818 2.0 9,410 5,147 (54.7) AL + 8 0
N789 2.5 5,472 5,472 (60.2) AL + 26 1
2) Cattle with > 100 proviral load
N787 N733 2.5 8,730 4,487 (51.4) AL + 294 250
2.5 8,230 6,313 (68.4) AL + 1,614 400
N823 2.0 9,340 6,239 (66.8) AL + 11,112 11,000
N788 2.5 9,620 5,435 (56.5) AL + 18,094 17,500
O10 15.0 12,200 7,900 (64.8) PL + 10,689 9,086
  1. aThe clinical stage of BLV infection was evaluated according to the lymphocyte count (per 1 μl), the detection of atypical mononuclear cells, and the age of the animal [19]. AL, BLV-infected but clinically and hematologically normal cattle; PL, BLV-infected but clinically normal cattle showing an increase in the number of apparently normal B lymphocytes. In the latter case, three separate lymphocyte counts were performed at different times and all yielded the same results.
  2. bELISA was performed using an anti-BLV ELISA kit according to the manufacturer’s instructions (JNC Inc., Tokyo, Japan). +, positive for anti-BLV antibodies; -, negative for anti-BLV antibodies.
  3. cThe proviral load (expressed as the copy number per 105 peripheral blood mononuclear cells [PBMCs]) was evaluated by BLV-CoCoMo-qPCR as previously described [19].
  4. dPBMCs were mixed with CC81 cells and 4 μg/ml polybrene (Sigma, St. Louis, MO) and used in the syncytium formation assay [19]. Syncytia were counted under a light microscope.