TY - JOUR AU - Panei, Carlos Javier AU - Takeshima, Shin-nosuke AU - Omori, Takashi AU - Nunoya, Tetsuo AU - Davis, William C. AU - Ishizaki, Hiroshi AU - Matoba, Kazuhiro AU - Aida, Yoko PY - 2013 DA - 2013/05/04 TI - Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR JO - BMC Veterinary Research SP - 95 VL - 9 IS - 1 AB - Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most common neoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistent lymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+ T cells, CD3+ T cells, CD4+ T cells, CD8+ T cells, γ/δ T cells, monocytes, and granulocytes in infected cattle that do not have tumors, although the most consistently infected cell is the CD5+ B cell. The mechanism by which BLV causes uncontrolled CD5+ B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chain reaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates not only with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The present study reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated from BLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR. SN - 1746-6148 UR - https://doi.org/10.1186/1746-6148-9-95 DO - 10.1186/1746-6148-9-95 ID - Panei2013 ER -