Table 2 Oligonucleotide primers designed for LAMP assays targeting the P32, RNA polymerase subunit RPO30 and DNA topoisomerase I
From: Detection of capripoxvirus DNA using a novel loop-mediated isothermal amplification assay
Target Region | Primer | Length | Position | Sequence (5′ – 3′) |
|---|---|---|---|---|
P32 | FIP | 58 | F1c 610-639 | TTCAAAACTCAAACTGGTAGAAATACCTTT- |
F2 559-582 | -GTAATTAGATTATCGTCTGCCATA | |||
BIP | 56 | B1c 667-696 | CTCAATAGACAAGTTTTAAATGACTCATCT- | |
B2 724-745 | -CGTTAGCTCTTTTTTTTGACAA | |||
F3 | 24 | 532-555 | GGATATGATTTTACCTTATCTGCA | |
B3 | 23 | 754-776 | CCAACTCTATTCCATATACCGTT | |
FLoop | 13 | 590-602 | ATAATTTCGTTTA | |
BLoop | 15 | 706-720 | CTTCACAATACTAAG | |
RNA Polymerase | FIP | 59 | F1c 300-331 | CTGTTCCATTTGTAGTACGTATAAGATTACAT- |
Subunit RPO30 | F2 244-266 | -ATTATCGTATAGAAACAAGCCTT | ||
BIP | 55 | B1c 437-468 | ATGTTTTAGATAAAAAGTATAACCTCCCATGC- | |
B2 487-505 | -TCATGACGGGAATAGTGTT | |||
F3 | 21 | 195-215 | TGAACCAAGAAACAACATAGG | |
B3 | 18 | 507-524 | TCTGCTGCTCTTGTTTGT | |
DNA | FIP | 54 | F1c 95-117 | GTGGGGCGGTATTCTAATCTTTT- |
Topoisomerase I | F2 56-81 | -CTCTTGTATCATTTGATAATCCTACT | ||
BIP | 52 | B1c 135-159 | TTACGAACAAACGTATGAACAGTCA- | |
B2 178-199 | -TACCTTTCGAATCTGAACCAAC | |||
F3 | 23 | 21-43 | CGATGGAAAACTTTTTACGGATA | |
B3 | 24 | 203-226 | GCAGTTTTCCATAAAAATATTGCC |