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Table 1 Diagnostic sensitivity and specificity of the CaPV LAMP assay as tested against representative strains of CaPV

From: Detection of capripoxvirus DNA using a novel loop-mediated isothermal amplification assay

Virus

Isolate name

LAMP result (Tp)a

PCR result (Ct)b

GTPV

Yemen

31.89

20.98

GTPV

Turkey

23.89

20.77

GTPV

Saudi Arabia

33.62

17.67

GTPV

Oman

27.17

22.96

GTPV

Mongolia

22.25

20.27

GTPV

India

22.65

19.44

GTPV

Held

29.81

24.01

GTPV

Gorgan (Iran)

34.34

21.97

GTPV

Ghana

38.10

26.05

GTPV

China

44.54

36.42

GTPV

Bangladesh

32.27

21.74

GTPV

Abu Ghraib (Iraq)

27.27

17.29

GTPV

Kano (Nigeria)

23.57

21.47

GTPV

Morocco

33.59

21.35

GTPV

Pakistan

44.96

40.28

GTPV

Vietnam

28.96

22.95

SP/GTPV

Kenya

29.62

18.22

SP/GTPV

Saudi Arabia

37.84

21.39

SP/GTPV

Nigeria

42.30

27.98

SP/GTPV

Yemen

29.56

22.75

SP/GTPV

KS-1

36.77

22.60

SPPV

Romania

37.14

24.78

SPPV

Abu Ghraib (Iraq)

34.91

21.84

SPPV

Isiolo (Kenya)

27.66

23.84

SPPV

Kedong (China)

24.83

21.75

SPPV

Mongolia

29.54

17.65

SPPV

Nigeria

36.19

29.03

SPPV

Oman

26.91

19.63

SPPV

Pakistan

38.79

22.60

SPPV

Senegal

40.02

21.71

SPPV

Cyprus

58.85

34.38

SPPV

India

41.68

23.52

SPPV

Sudan

32.35

21.03

SPPV Vaccine Strain

Saudi Arabia

38.05

22.89

SPPV Vaccine Strain

Stavropol (Russia)

37.61

20.79

SPPV Vaccine Strain

France

38.33

21.62

SPPV Vaccine Strain

Pendik (Turkey)

36.38

22.27

LSDV

Israel

27.75

20.96

LSDV

Nigeria

30.16

21.82

LSDV

Uganda

23.73

18.69

LSDV

Senegal

29.22

23.26

LSDV

Cameroon

31.49

21.50

LSDV

Egypt 93

31.41

23.37

LSDV

Egypt 2006

32.14

26.09

LSDV

Neethling

26.98

19.59

LSDV

Oman

32.19

32.19

LSDV

Ghana

28.55

22.63

n/a

Negative Cattle Blood (n = 14)

Negative (n = 14)

Negative (n = 14)

n/a

Negative Sheep Blood (n = 8)

Negative (n = 8)

Negative (n = 8)

n/a

Negative Cattle Skin (n = 16)

Negative (n = 16)

Negative (n = 16)

Buffalopox virus

n/a

Negative

Not Tested

Camelpox virus

n/a

Negative

Not Tested

Cheetah poxvirus

n/a

Negative

Not Tested

Cowpox virus

n/a

Negative

Not Tested

Orf virus

n/a

Negative

Not Tested

Swine poxvirus

n/a

Negative

Not Tested

  1. a T p  = Time to positive (minutes).
  2. b C t  = threshold cycle value.