Evaluation of candidate LAMP assays. A: Agarose gel (2%) showing the characteristic laddering pattern generated by LAMP for the detection of GTPV isolate Vietnam Ninh Tuan 05 by three different LAMP primer sets: EP32A (P32 gene); Epol (RNA polymerase RPO30 subunit) and ETop (DNA topoisomerase I). Results for negative controls (−) are shown. MW: molecular weight ladder (100 bp). B: Corresponding increase in fluorescence generated for EP32A (○: circle); Epol (△: triangle) and ETop (◊: diamond) LAMP assays using a real-time PCR machine. Representative signal for a negative control (□) well is shown.