|
Fraction used for VVA chromatography
|
PAGa/TPbratio
|
MMcof major stained bands
|
N-terminal amino acid sequence
|
Code of protein
|
Accession number
|
|---|
| | |
Coomassie staining
|
Western blot
| | | |
|---|
|
D40-G75 peak III
|
> 100%
|
69
|
64
|
RGSXLTIHP
|
wbPAG69kDa_A
|
P86372
|
|
D80-G75 peak I
|
65.8%
|
73
|
68
|
RGSXLTILPLRNKIDLFYVG
|
wbPAG73kDa_B
|
P86373
|
| | |
61
|
59
|
RGSXLTILPLRNIRDIFYVG
|
wbPAG61kDa_C
|
P86374
|
|
D80-G75 peak II
|
> 100%
|
76
|
70
|
RGSXLTIHPL
|
wbPAG76kDa_D
|
P86375
|
| | |
65
|
60
|
RGSXLTH
|
wbPAG65kDa_E
|
P86376
|
| | |
58
|
55
|
RGSXLTHLP
|
wbPAG58kDa_F
|
P86377
|
|
D160-G75 peak I
|
> 100%
|
76
|
70
|
RGSXLTIHP
|
wbPAG76kDa_G
|
P86378
|
| | |
63
|
60
|
RGSXLTIHP
|
wbPAG63kDa_H
|
P86379
|
|
D160-G75 peak II
|
> 100%
|
73
|
68
|
RGSXLTHLPLRNISD
|
wbPAG73kDa_I
|
P86371
|
| | |
63
|
61
|
RGSXLTILPLRNISD
|
wbPAG63kDa_J
|
P86370
|
| | |
60
|
57
|
RGSXLTIHPL
|
wbPAG60kDa_K
|
P86369
|
- aDetermined by radioimmunoassay. bObtained by weighing each fraction. cApparent molecular masses (MM) were estimated after SDS-PAGE and Western blot (AS#497 + AS#708, 0.1:0.1 ml, v/v).
