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Table 2 Characteristics of different eluted fractions giving the highest ratio of PAG/TP obtained after chromatography on Vicia villosa agarose

From: Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalisplacentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Fraction used for VVA chromatography PAGa/TPbratio MMcof major stained bands N-terminal amino acid sequence Code of protein Accession number
   Coomassie staining Western blot    
D40-G75 peak III > 100% 69 64 RGSXLTIHP wbPAG69kDa_A P86372
D80-G75 peak I 65.8% 73 68 RGSXLTILPLRNKIDLFYVG wbPAG73kDa_B P86373
   61 59 RGSXLTILPLRNIRDIFYVG wbPAG61kDa_C P86374
D80-G75 peak II > 100% 76 70 RGSXLTIHPL wbPAG76kDa_D P86375
   65 60 RGSXLTH wbPAG65kDa_E P86376
   58 55 RGSXLTHLP wbPAG58kDa_F P86377
D160-G75 peak I > 100% 76 70 RGSXLTIHP wbPAG76kDa_G P86378
   63 60 RGSXLTIHP wbPAG63kDa_H P86379
D160-G75 peak II > 100% 73 68 RGSXLTHLPLRNISD wbPAG73kDa_I P86371
   63 61 RGSXLTILPLRNISD wbPAG63kDa_J P86370
   60 57 RGSXLTIHPL wbPAG60kDa_K P86369
  1. aDetermined by radioimmunoassay. bObtained by weighing each fraction. cApparent molecular masses (MM) were estimated after SDS-PAGE and Western blot (AS#497 + AS#708, 0.1:0.1 ml, v/v).