Skip to main content

Table 2 Characteristics of different eluted fractions giving the highest ratio of PAG/TP obtained after chromatography on Vicia villosa agarose

From: Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalisplacentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Fraction used for VVA chromatography

PAGa/TPbratio

MMcof major stained bands

N-terminal amino acid sequence

Code of protein

Accession number

  

Coomassie staining

Western blot

   

D40-G75 peak III

> 100%

69

64

RGSXLTIHP

wbPAG69kDa_A

P86372

D80-G75 peak I

65.8%

73

68

RGSXLTILPLRNKIDLFYVG

wbPAG73kDa_B

P86373

  

61

59

RGSXLTILPLRNIRDIFYVG

wbPAG61kDa_C

P86374

D80-G75 peak II

> 100%

76

70

RGSXLTIHPL

wbPAG76kDa_D

P86375

  

65

60

RGSXLTH

wbPAG65kDa_E

P86376

  

58

55

RGSXLTHLP

wbPAG58kDa_F

P86377

D160-G75 peak I

> 100%

76

70

RGSXLTIHP

wbPAG76kDa_G

P86378

  

63

60

RGSXLTIHP

wbPAG63kDa_H

P86379

D160-G75 peak II

> 100%

73

68

RGSXLTHLPLRNISD

wbPAG73kDa_I

P86371

  

63

61

RGSXLTILPLRNISD

wbPAG63kDa_J

P86370

  

60

57

RGSXLTIHPL

wbPAG60kDa_K

P86369

  1. aDetermined by radioimmunoassay. bObtained by weighing each fraction. cApparent molecular masses (MM) were estimated after SDS-PAGE and Western blot (AS#497 + AS#708, 0.1:0.1 ml, v/v).