|
Protein extraction
|
140,897.6
|
5,719.5
|
4.1
|
|
Acid precipitation
|
55,598.0
|
2,919.5
|
5.2
|
|
Ammonium sulfate 40–80%
|
19,828.5
|
3,025.5
|
15.3
|
|
DEAE 40 mM NaCl (D40)a
|
1,132.1
|
176.3
|
15.6
|
|
Sephadex G75-D40 peak I (tubes 36–39)
|
67.2
|
9.4
|
14.8
|
|
Sephadex G75-D40 peak II (tubes 40–43)
|
82.2
|
24.3
|
29.6
|
|
Sephadex G75-D40 peak III (tubes 44–47)
|
39.0
|
17.0
|
43.6
|
|
DEAE 80 mM NaCl (D80)a
|
2,481.3
|
824.2
|
33.2
|
|
Sephadex G75-D80 peak I (tubes 36–42)
|
146.4
|
95.4
|
65.1
|
|
Sephadex G75-D80 peak II (tubes 43–49)
|
129.8
|
74.7
|
57.5
|
|
DEAE 160 mM NaCl (D160)a
|
3,527.7
|
174.6
|
4.9
|
|
Sephadex G75-D160 peak I (tubes 37–38)
|
74.4
|
16.2
|
21.7
|
|
Sephadex G75-D160 peak II (tubes 39)
|
31.5
|
7.7
|
24.4
|
