Table 1 Total protein, immunoreactive PAG and ratio of immunoreactive PAG/total protein in different steps of purification

Protein extraction

140,897.6

5,719.5

4.1

Acid precipitation

55,598.0

2,919.5

5.2

Ammonium sulfate 40–80%

19,828.5

3,025.5

15.3

DEAE 40 mM NaCl (D40)^a

1,132.1

176.3

15.6

 Sephadex G75-D40 peak I (tubes 36–39)

67.2

9.4

14.8

 Sephadex G75-D40 peak II (tubes 40–43)

82.2

24.3

29.6

 Sephadex G75-D40 peak III (tubes 44–47)

39.0

17.0

43.6

DEAE 80 mM NaCl (D80)^a

2,481.3

824.2

33.2

 Sephadex G75-D80 peak I (tubes 36–42)

146.4

95.4

65.1

 Sephadex G75-D80 peak II (tubes 43–49)

129.8

74.7

57.5

DEAE 160 mM NaCl (D160)^a

3,527.7

174.6

4.9

 Sephadex G75-D160 peak I (tubes 37–38)

74.4

16.2

21.7

 Sephadex G75-D160 peak II (tubes 39)

31.5

7.7

24.4