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Table 1 Total protein, immunoreactive PAG and ratio of immunoreactive PAG/total protein in different steps of purification

From: Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalisplacentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Purification steps TP (mg) equivPAG (mg) PAG/TP ratio (%)
Protein extraction 140,897.6 5,719.5 4.1
Acid precipitation 55,598.0 2,919.5 5.2
Ammonium sulfate 40–80% 19,828.5 3,025.5 15.3
DEAE 40 mM NaCl (D40)a 1,132.1 176.3 15.6
 Sephadex G75-D40 peak I (tubes 36–39) 67.2 9.4 14.8
 Sephadex G75-D40 peak II (tubes 40–43) 82.2 24.3 29.6
 Sephadex G75-D40 peak III (tubes 44–47) 39.0 17.0 43.6
DEAE 80 mM NaCl (D80)a 2,481.3 824.2 33.2
 Sephadex G75-D80 peak I (tubes 36–42) 146.4 95.4 65.1
 Sephadex G75-D80 peak II (tubes 43–49) 129.8 74.7 57.5
DEAE 160 mM NaCl (D160)a 3,527.7 174.6 4.9
 Sephadex G75-D160 peak I (tubes 37–38) 74.4 16.2 21.7
 Sephadex G75-D160 peak II (tubes 39) 31.5 7.7 24.4
  1. aEight hundred milligrams from each DEAE were loaded on the Sephadex G75.
  2. TP: total protein.
  3. equivPAG: PAG concentrations measured by heterologous RIA.