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Figure 3 | BMC Veterinary Research

Figure 3

From: Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalisplacentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females

Figure 3

Coomassie blue stained PVDF membrane after SDS-PAGE (A) and Western blot analysis with AS#708 (B). Lane 1: VVA peak III from the 40 mM NaCl-DEAE fraction. Lane 2: VVA peak I from the 80 mM NaCl-DEAE fraction. Lane 3: VVA peak II from the 80 mM NaCl-DEAE fraction. Lane 4: VVA peak I from the 160 mM NaCl-DEAE fraction. Lane 5: VVA peak II from the 160 mM NaCl-DEAE fraction. Thirty (A) to 50 μg (B) were loaded in lanes 1–5. Molecular weight standards (kDa; 7 μg/lane) were loaded on the left and the right sides of each figure. Final dilution of PAG AS#708 was 1:2,000.

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