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Table 4 mAbs used to identify leukocyte subsets in hemal and retropharyngeal lymph nodes during immunohistochemistry study

From: Accumulation profiles of PrPScin hemal nodes of naturally and experimentally scrapie-infected sheep

Cell types

Cell surface marker

mAb clones

Species and isotypes

Dilution (μg/mL)

Source

T ymphocytes

CD3

A0452

Rabbit poly

3.0

Dako

B lymphocytes

CD79a

MCA2538

mouse IgG1

50.0

AbD Serotec

FDCs

120 kDa

CAN.42

mouse IgM

4.1

Dako

Mac

CD163

MCA1853

mouse IgG1

33.3

AbD Serotec

  1. Abbreviations, mAb = monoclonal antibody; poly = polyclonal antibody; FDCs = follicular dendritic cells; Mac = macrophages.