Figure 3From: Exposure of RML scrapie agent to a sodium percarbonate-based product and sodium dodecyl sulfate renders PrPScprotease sensitive but does not eliminate infectivityWestern blot of PrPScin brain homogenate from RML scrapie-affected C57Bl/6 mice treated with 0.35 M sodium hydrogen phosphate buffered solution (pH 11) for 30 (lanes 3, 8), 90 (lanes 4, 9), or 180 minutes (lanes 5, 10). PrPSc-immunoreactivity was undetectable after PK digestion (lanes 3β5). PK digestion was omitted prior to immunoblotting for samples in lanes 7β10. Lanes 1 and 6, molecular weight marker (kDa); lanes 2 and 7, RML positive control.Back to article page