Western blot of PrPScin brain homogenate from RML scrapie-affected C57Bl/6 mice treated with SPC-P
alone (A) or in combination with 2.5% SDS (B). A) PrPSc was still detectable after PK digestion of samples exposed to SPC-PL (lanes 3–5), but not in samples treated with SPCH (lanes 7–9). B) PrPSc immunoreactivity was undetectable after PK digestion of samples exposed to either SPC-PL + SDS (lanes 3–5) or SPC-PH + SDS (lanes 7–9). Lane 1, molecular weight marker; lane 2, RML positive control; lane 6, empty. Abbreviations: MW, molecular weight; SPC-PL or H, low or high concentration sodium percarbonate-based product; SDS, sodium dodecyl sulfate; m, minutes.