Figure 3From: Gene expression profiles in canine mammary carcinomas of various grades of malignancyThe canine mammary carcinoma tissues which pathological diagnosis differed from molecular classification. The pictures of canine mammary carcinoma tissues (haematoxylin-eosin staining) which pathological diagnosis differed from their molecular classification. A. Tumour no 9. (pathologically grade 2 complex carcinoma, classified to the most malignant group). Moderate tubule formation was observed (×200). a) Neoplastic cells exhibited moderate nuclear pleomorphism, mild to moderate hyperchromasia with noticeable nucleoli was observed. Eight mitoses per 10 HPF were present. Mitotic cell is indicated by the arrow (400×). B. Tumour no. 88 (pathologically grade 1 complex carcinoma, classified to the third group). Moderate tubule formation was observed. Epithelial cells were arranged in irregular tubules lined by a single to few layers of cells. Some tubules contained an eosinophilic secretion (200×). b) Neoplastic epithelial cells had regular small nuclei (round to ovoid) with small or indistinct nucleoli. Presence of 1 mitose per 10 HPF. Mitotic cell was indicated by the arrow (400×). C. Tumour no. 72: pathologically grade 3 simple carcinoma, classified to the third group. A few tubules were observed. In some areas neoplastic cells were closely packed and arranged in solid foci (100×). c) Marked nuclear pleomorphism was observed as well as cells containing stippled chromatin and variably distinct nucleoli. Twenty five mitoses per 10 HPF were counted (arrows). (400×). D. Tumour no. 67: pathologically grade 3 complex carcinoma, classified to the third group. Moderate tubule formation was observed (200×). d) Marked nuclear pleomorphism and presence of nuclei with hyperchromasia were observed. Twenty eight mitoses per 10 HPF were noted (arrows) (400×).Back to article page