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Table 2 Mean fluorescence related to CSF-1R expression

From: CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells

  ctrl Without FBS csf-1rsiRNA Non-coding siRNA CSF-1 25 ng/ml CSF-1 50 ng/ml CSF-1 100 ng/ml
CMT-U27 284.0 191.0* 76.00** 306.5 2450*** 2430*** 2616***
CMT-U309 255.0 217.5* 107.0*** 251.0 5114*** 4080*** 4100***
P114 458.5 200.0* 48.50** 537.0 4680*** 4638*** 6178***
CMT-W1 335.0 190.0* 218.5* 383.5 1861** 2268** 1780**
CMT-W2 195.5 130.8* 80.50* 228.5 2448*** 2200*** 2679***
  1. Mean fluorescence related to CSF-1R expression obtained with FACS Aria II (Becton Dickinson). Mean fluorescence of CSF-1R is significantly decreased after the csf-1r specific siRNA treatment and significantly increased after treatment with CSF-1 at the dose of 25, 50 and 100 ng/ml (no significant differences between these doses have been observed). Treatment of cells with non-coding siRNA did not cause any effect. The experiment has been conducted in three replicates. P<0.05 was regarded as significant and marked as *, whereas p<0.01 and p<0.001 was regarded as highly significant and marked as * and ***, respectively. One-way ANOVA followed by Tukey HSD post-hoc test were applied.