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Table 1 Number of cells (%) expressing CSF-1R

From: CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells

  ctrl Without FBS csf-1r siRNA Non-coding siRNA CSF-1 25 ng/ml CSF-1 50 ng/ml CSF-1 100 ng/ml
CMT-U27 72.10 73.4 15.65*** 75.80 99.85*** 99.80*** 99.65***
CMT-U309 66.80 70.0 3.00*** 64.40 99.75* 99.85* 99.65*
P114 88.70 73.7 38,45*** 90.85 99.80* 99.80* 99.90*
CMT-W1 80.45 73.6 23.45*** 82.30 99.55** 99.55** 99.75**
CMT-W2 58.70 52.3 23.15*** 64.80 99.35*** 99.10*** 99.55***
  1. The number of CSF-1R expressing cells obtained with FACS Aria II (Becton Dickinson). The number of cells which express CSF-1R is significantly decreased after the csf-1r specific siRNA treatment and significantly increased after treatment with CSF-1 at the dose of 25, 50 and 100 ng/ml (no significant differences between these doses have been observed). Treatment of cells with non-coding siRNA did not cause any effect. The experiment has been conducted in three replicates. P<0.05 was regarded as significant and marked as *, whereas p<0.01 and p<0.001 was regarded as highly significant and marked as * and ***, respectively. One-way ANOVA followed by Tukey HSD post-hoc test were applied.