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Figure 4 | BMC Veterinary Research

Figure 4

From: CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells

Figure 4

Examination of CSF-1R activation by Immunohistochemistry. A. The graph of mean optical density of p-CSF-1R (Tyr 723) in canine mammary cell lines (CMT-U27, CMT-U309, P114, CMT-W1 and CMT-W2) grown in control conditions (ctrl), serum-starving conditions (−FBS) and in serum-starving conditions and supplemented with CSF-1 (25 ng/ml) (−FBS/+CSF-1 25 ng/ml). B. Representative pictures of p-CSF-1R expression in CMT-W1 cell line (in control conditions, serum-starving conditions and serum starving conditions and supplemented with CSF-1 (25 ng/ml) obtained using Olympus BX60 microscope (at the magnification of 200x). The p-CSF-1R is reflected as brown precipitate. Ten pictures in each slide were analyzed. The colorimetric intensity of the IHC-stained antigen spots was counted by a computer-assisted image analyzer (Olympus Microimage™ Image Analysis, software version 4.0 for Windows, USA) and the antigen spot color intensity is expressed as mean pixel integrated optical density (IOD). The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). The ANOVA + Tukey post-hoc tests were applied to analyze the optical density in cell lines. p<0.05 was regarded as significant and marked as *, whereas values differed highly significant (p≤0.01 and p≤0.001) were marked as ** or ***, respectively.

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