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Table 4 Summary of porcine reproductive and respiratory syndrome virus (PRRSV) serum and oral fluid antibody enzyme linked-immunosorbent assay (ELISA) condition a

From: Kinetics of the porcine reproductive and respiratory syndrome virus (PRRSV) humoral immune response in swine serum and oral fluids collected from individual boars

  IgM IgA IgG Commercial ELISA
Serum ELISAs     
  Sample dilution 1:40 1:5 1:40 1:40
  Sample volume 100 μl 100 μl 100 μl 100 μl
  Conjugate dilution 1:5,000b 1:1,000c 1:15,000d Provided with kit
  Negative control 100 μl of pooled negative serum diluted 1:40 100 μl of pooled negative serum diluted 1:5 100 μl of kit negative control 100 μl of kit negative control
  Positive control 100 μl of pooled serum from DPI 7 diluted 1:40 100 μl of pooled serum from DPI 21 diluted 1:5 100 μl of kit positive control 100 μl of kit positive control
Oral fluid ELISAs     
  Sample dilution 1:2 1:2 1:2  
  Sample volume 250 μl 250 μl 250 μl  
  Conjugate dilution 1:3,800b 1:2,000c 1:2,400d  
  Negative control 250 μl of reference standard oral fluide DPI 0 diluted 1:2 250 μl of reference standard oral fluide DPI 0 diluted 1:2 100 μl of kit negative control diluted 1:30  
  Positive control 250 μl of reference standard oral fluide DPI 10diluted 1:5 250 μl of reference standard oral fluide DPI 91 diluted 1:2 100 μl of kit positive control diluted 1:30  
  1. a Oral fluid ELISA conditions represent modifications to a commercial PRRSV serum antibody ELISA protocol (IDEXX PRRS X3 Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA).
  2. b Anti-pig IgM: HRPO conjugate (A100-100P, Bethyl Laboratories, Montgomery, TX, USA).
  3. c Anti-pig IgA: HRPO conjugate (A100-102P, Bethyl Laboratories, Montgomery, TX, USA).
  4. d Anti-pig IgGFc: HRPO conjugate (A100-104P, Bethyl Laboratories, Montgomery, TX, USA).
  5. e Reference standard oral fluid samples have been previously described [5].