Figure 4From: Kinetics of the porcine reproductive and respiratory syndrome virus (PRRSV) humoral immune response in swine serum and oral fluids collected from individual boarsCalculation of the optimal dilution of anti-pig secondary antibody for PRRSV IgM, IgA, IgG ELISAs. The relationship between the positive control optical density (OD) and dilution of anti-pig secondary antibody was plotted as y = ax + c, where (y) is the secondary antibody OD response, (a) is the slope of the line, (x) is the dilution of secondary antibody, and (c) is the intercept. The appropriate dilution corresponds to the positive control value provided in the Certificate of Analysis.Back to article page