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Table 3 Single and multiplex PCR analytical sensitivity in semen samples spiked with 100to 106CFU/mL of Brucella ovis , Atinobacillus seminis or Histophilus somni

From: Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams

  

CFU/mL of semen

  

100

101

102

103

104

105

106

Single PCR

B. ovis

-

-

-

-

+

+

+

 

A. seminis

-

+

+

+

+

+

+

 

H. somni

-

-

-

+

+

+

+

Multiplex PCR

B. ovis

-

-

-

-

+

+

+

 

A. seminis

-

-

+

+

+

+

+

 

H. somni

-

-

-

-

-

-

-

  1. + : positive; -: negative.