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Table 2 ELISA test parameters when using protein G or anti-deer conjugates with elk serum

From: The modification and evaluation of an ELISA test for the surveillance of Mycobacterium avium subsp. paratuberculosisinfection in wild ruminants

 

S/P cut-off

AUC [95% CI]

Se [95% CI]

Sp [95% CI]

PPV [95% CI]

NPV [95% CI]

Protein G

0.22

0.81 [0.65; 0.91]

73% [50; 89]

90% [68; 99]

89% [65; 99]

75% [53; 90]

Anti-deer

0.29

0.85 [0.71; 0.95]

68% [45; 86]

100% [83; 100]

100% [78; 100]

74% [54; 89]

  1. Sample-to-positive (S/P) ratio cut-off values as determined by Receiver Operational Curve (ROC) analysis, the area under the curve (AUC), and the resulting sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV).