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Table 1 Checkerboard test results

From: The modification and evaluation of an ELISA test for the surveillance of Mycobacterium avium subsp. paratuberculosisinfection in wild ruminants

  Elk Caribou Bison
Protein G dilution (a) 1:16000 1:8000 1:16000
Anti-deer dilution 1:250 (b) 1:175 (c) NT
Sample dilution using Protein G 1:30 1:20 1:160
Sample dilution using anti-deer 1:30 1:5 NT
  1. (a) Concentration not available from manufacturer.
  2. (b) 0.40 μg/ml.
  3. (c) 0.57 μg/ml.
  4. Optimal dilutions of Protein G and anti-deer conjugates when used with serum from elk, caribou or bison, and optimal species-specific serum dilutions for use with the IDEXX Mycobacterium paratuberculosis Antibody Test. NT: not tested.