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Table 1 Checkerboard test results

From: The modification and evaluation of an ELISA test for the surveillance of Mycobacterium avium subsp. paratuberculosisinfection in wild ruminants

 

Elk

Caribou

Bison

Protein G dilution (a)

1:16000

1:8000

1:16000

Anti-deer dilution

1:250 (b)

1:175 (c)

NT

Sample dilution using Protein G

1:30

1:20

1:160

Sample dilution using anti-deer

1:30

1:5

NT

  1. (a) Concentration not available from manufacturer.
  2. (b) 0.40 μg/ml.
  3. (c) 0.57 μg/ml.
  4. Optimal dilutions of Protein G and anti-deer conjugates when used with serum from elk, caribou or bison, and optimal species-specific serum dilutions for use with the IDEXX Mycobacterium paratuberculosis Antibody Test. NT: not tested.
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BMC Veterinary Research

ISSN: 1746-6148

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