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Table 3 Follicle distribution in ovarian cortex pieces from an Amur leopard of 14 days in vitro culture with A) FCS and B) BSA supplementation

From: Short-term culture of ovarian cortex pieces to assess the cryopreservation outcome in wild felids for genome conservation

A) Mean number (%)
Follicle group Day 0 Day 2 Day 7 Day 14
  (n = 2) (n = 3) (n = 3) (n = 3)
all follicles 313 ± 123 98 ± 69 62 ± 16 55 ± 4
shrunken/truncated follicles 29 ± 9 (9,24%) 20 ± 11 (25,97%) 18 ± 7 (28,45%) 47 ± 6 (85,41%)
primordial follicles (%PF) 129 ± 57 (40,82%) 43 ± 33 (38,54%) 29 ± 8 (46,27%) 6 ± 4 (11,43%)
primary follicles 107 ± 37 (34,32%) 21 ± 19 (16,77%) 9 ± 3 (15,08%) 1 ± 1 (1,89%)
secondary follicles 49 ± 20 (15,62%)a 14 ± 8 (18,73%)a,b 7 ± 8 (10,20%)a,b 1 ± 1 (1,27%)b
%PF40 (measured follicles) 40,00% (80) 42,58% (102) 45,83% (120) 11,67% (120)
CFN 174 87 72 8
B) Mean number (%)
Follicle group Day 0 Day 2 Day 7 Day 14
  (n = 2) (n = 3) (n = 3) (n = 2)
all follicles 313 ± 123a 145 ± 23a,b 103 ± 35a,b 24 ± 28b
shrunken/truncated follicles 29 ± 9 (9,24%) 65 ± 11 (44,55%) 52 ± 20 (49,49%) 8 ± 11 (17,05%)
primordial follicles (%PF) 129 ± 57 (40,82%)a 43 ± 8 (29,59%)a,b 32 ± 15 (30,35%)a,b 10 ± 9 (55,68%)b
primary follicles 107 ± 37 (34,32%) 17 ± 5 (11,53%) 11 ± 3 (10,98%) 6 ± 8 (13,64%)
secondary follicles 49 ± 20 (15,62%)a 21 ± 3 (14,33%)a,b 9 ± 2 (9,08%)a,b 1 ± 0 (13,64%)b
%PF40 (measured follicles) 40,00% (80) 33,33% (120) 24,17% (120) 55,00% (44)
CFN 174 67 43 31
  1. a, b - different subscript indicate for significant differences.