Table 3 Follicle distribution in ovarian cortex pieces from an Amur leopard of 14 days in vitro culture with A) FCS and B) BSA supplementation

all follicles

313 ± 123

98 ± 69

62 ± 16

55 ± 4

shrunken/truncated follicles

29 ± 9 (9,24%)

20 ± 11 (25,97%)

18 ± 7 (28,45%)

47 ± 6 (85,41%)

primordial follicles (%PF)

129 ± 57 (40,82%)

43 ± 33 (38,54%)

29 ± 8 (46,27%)

6 ± 4 (11,43%)

primary follicles

107 ± 37 (34,32%)

21 ± 19 (16,77%)

9 ± 3 (15,08%)

1 ± 1 (1,89%)

secondary follicles

49 ± 20 (15,62%)^a

14 ± 8 (18,73%)^a,b

7 ± 8 (10,20%)^a,b

1 ± 1 (1,27%)^b

%PF₄₀ (measured follicles)

40,00% (80)

42,58% (102)

45,83% (120)

11,67% (120)

CFN

174

87

72

8

B)

Mean number (%)

Follicle group

Day 0

Day 2

Day 7

Day 14

(n = 2)

(n = 3)

(n = 3)

(n = 2)

all follicles

313 ± 123^a

145 ± 23^a,b

103 ± 35^a,b

24 ± 28^b

shrunken/truncated follicles

29 ± 9 (9,24%)

65 ± 11 (44,55%)

52 ± 20 (49,49%)

8 ± 11 (17,05%)

primordial follicles (%PF)

129 ± 57 (40,82%)^a

43 ± 8 (29,59%)^a,b

32 ± 15 (30,35%)^a,b

10 ± 9 (55,68%)^b

primary follicles

107 ± 37 (34,32%)

17 ± 5 (11,53%)

11 ± 3 (10,98%)

6 ± 8 (13,64%)

secondary follicles

49 ± 20 (15,62%)^a

21 ± 3 (14,33%)^a,b

9 ± 2 (9,08%)^a,b

1 ± 0 (13,64%)^b

%PF₄₀ (measured follicles)

40,00% (80)

33,33% (120)

24,17% (120)

55,00% (44)

CFN

174

67

43

31