Table 3 Primers and probes used in real-time PCR analysis
Primer name | Sequence (5’to 3’) | Detection |
|---|---|---|
WS440570.1-F | CAA CTA GCA AGC ACT GGT ATT TTC AAT AA | |
WS440570.1-R | CGG CCA ATA AAT CTG CAG TCA TT | WSSV |
WS440570.1-Probe | CAA CAT GCC AGT TTT C | |
IH273215-F | ACT ACG ACA ATA TGC AGT GGA TAA AAT ACA | |
IH273215-R | GTC TGC TAC GAT GAT TAT CCA AGC T | IHHNV |
IH273215-Probe | CCG TGT ACC AGA AAT C | |
EF021452-F | CGT GAA GAA CGT GTC TGT AAA GGA T | |
EF021452-R | GGC TGG GTC GTT CTT CGA | Shrimp elongation factor |
EF021452-Probe | AAG CGA CGA ATC CAC |