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Table 2 Strain typing of 171 selected Staphylococcus aureus isolates from 2 herds 1

From: Effect of lactation therapy on Staphylococcus aureus transmission dynamics in two commercial dairy herds

Strain type IMI5 Herd In vitro Antimicrobial Susceptibility-Minimum Inhibitory Concentration values, MIC50/MIC90(ug/ml)6 antibiotic resistance PCR blaZ/mecA
PT2 ST3 MLST CC4    Amp Ceph Ceftio Ery Oxa Pen Pen: Novo Pirl Tet  
1 151 705 3 2 ≤0.12/0.25 ≤2/≤2 ≤0.5/1 ≤0.25/≤0.25 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/1 ≤1/≤1 −/−
  2185 705 22 1 ≤0.12/0.25 ≤2/≤2 ≤0.5/4 ≤0.25/≤0.5 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/≤0.5 ≤1/2 −/−
1a 2185 705 1 1 ≤0.12 ≤2 1 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1 −/−
1b 2185 705 6 1 ≤0.12/≤0.12 ≤2/≤2 ≤0.5/2 ≤0.25/≤0.25 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/≤0.5 ≤1/≤1 −/−
2185 705 3 1 ≤0.12/≤0.12 ≤2/≤2 1/1 ≤0.25/≤0.25 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/≤0.5 ≤1/≤1 −/−
1c nd   1 1 ≤0.12 ≤2 1 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 4 −/−
2 2189 97 5 1 ≤0.12/≤0.12 ≤2/8 1/1 ≤0.25/1 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/1 ≤1/2 −/−
2189 97 7 1 ≤0.12/≤0.12 ≤2/≤2 1/1 ≤0.25/≤0.25 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/1 ≤1/≤1 −/−
2a nd   1 1 ≤0.12 ≤2 2 ≤0.25 ≤2 ≤0.12 ≤(1:2) 2 4 −/−
2b nd   4 1 ≤0.12/≤0.12 ≤2/≤2 1/1 ≤0.25//≤0.25 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/2 2/4 −/−
3 8 5 7 2 0.5/0.5 ≤2/≤2 1/1 ≤0.25/≤0.25 ≤2/≤2 0.25/0.5 ≤(1:2)/≤(1:2) ≤0.5/≤0.5 ≤1/≤1 +/−
4 352 97 2 2 ≤0.12 ≤2 2 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1/≤1 −/−
4a 352 97 5 2 ≤0.12/≤0.12 ≤2/4 1/1 0.5/0.5 ≤2/≤2 ≤0.12/≤0.12 ≤(1:2)/≤(1:2) ≤0.5/≤0.5 ≤1/≤1 −/−
4bfh nd   1 1 ≤0.12 ≤2 1 0.5 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1/≤1 −/−
5 2187 97 1 1 ≤0.12 ≤2 1 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1 −/−
5a nd   1 1 ≤0.12 ≤2 1 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1 −/−
6 nd   1 1 ≤0.12 ≤2 ≤0.5 ≤0.25 ≤2 ≤0.12 ≤(1:2) ≤0.5 ≤1 −/−
7 20 20 1 2 ≤0.12 ≤2 ≤0.5 ≤0.25 ≤2 0.25 ≤(1:2) ≤0.5 ≤1 −/−
8 479 479 1 1 ≤0.12 ≤2 ≤0.5 ≤0.25 ≤2 ≤0.12 2/4 ≤0.5 ≤1 −/−
9 87 59 0 1 8 4 2 8 4 8 ≤(1:2) 1 2 +/+
10 25 15 1 3 16 ≤2 1 ≤0.25 ≤2 8 ≤(1:2) ≤0.5 ≤1 +/−
11 nd   0 2 ≤0.25 ≤2 ≤0.5 ≤0.25 ≤2 0.25 ≤(1:2) ≤0.5 ≤1 −/−
  1. 1 Isolates from 74 IMI including 61 IMI identified in cows in treatment and control pens and13 IMI identified in cows that did not enter study pens. An additional 10 incidental isolates were also typed.
  2. 2 PFGE pulsotypes; types with the same number but different letters are minor variants with 1–3 band differences.
  3. 3 Sequence type determined by MLST.
  4. 4 MLST clonal complex predicted founder determined by eBurst MLST database (last searched May 30, 2012).
  5. 5 Number of quarters with IMI for each strain type.
  6. 6 Antimicrobial susceptibility for ampicillin (Amp), cephalothin (Ceph), ceftiofur (Ceftio), erythromycin (Ery), oxacillin (Oxa), penicillin (Pen), penicillin:novobiocin (Pen:Novo), pirlimycin (Pirl), and tetracycline (Tet); MIC50 and MIC90 values calculated as described in [25].
  7. † type found as only an incidental isolate, not defined as IMI or associated with subclinical mastitis (SCC< 200,000 cells/ml).
  8. fh type found in fresh heifer with clinical mastitis, cow did not enter study pens.
  9. nd = not determined.