Table 1 Designed primers used for PCR and DNA sequencing (Eurogentec S.A., Belgium)
Primer | Sequence (5′→3′) | Position (relative to human BSEP [NM_003742]) |
|---|---|---|
Oligo(dT)-anchor | 5′-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTT | |
PCR-anchor | 5′-GACCAGGCGTATCGATGTCGAC | |
BSEP_RACE_A | 5′-AGTGTTGTTTGCCTGTAGC | 3615-3633 |
BSEP_RACE_B | 5′-TGAAGAAAATCGAAACAAT | 273-291 |
BSEP_RACE_C | 5′-TGTGCCAAAAATGAGGAG | 367-381 |
BSEP_RACE_D | 5′-AGTTCTTGTAATTCAGTGTCA | 405-425 |
BSEP_Af | 5′-CTCGACCTGATACGCAAGTTCTGA | 3392-3415 |
BSEP_Ar | 5′-AATGGCCCGAGCAATAGCAATAC | 3805-3825 |
BSEP_Bf | 5′-CAAGGGAAGGTGATGATAGATGG | 3526-3548 |
BSEP_Br | 5′-GATGGGGGCTCCTGTGGTAACT | 4065-4086 |
BSEP_Cf | 5′-TGTGCTTCTTCCCCTTCTTGGCT | 2840-2862 |
BSEP_Cr | 5′-TGCCCATCTATCATCACCTTCC | 3530-3548 |
BSEP_Df | 5′-CAACGCTCCAAGTCTCA | 2215-2231 |
BSEP_Dr | 5′-GTGCGGATATTACTGAG | 2956-2972 |
BSEP_Ef | 5′-CAAGGCTTGCTACGGATGC | 2702-2720 |
BSEP_Er | 5′-TGATTGGGGGTTGTCGATC | 3295-3310 |
BSEP_Ff | 5′-GTGGTGGCCAGAAACAAAG | 1805-1823 |
BSEP_Fr | 5′-TCTGGCTGAATAAAAAGGCA | 2442-2461 |
BSEP_Gf | 5′-TGGATCGAATTAAGGGTGAA | 1364-1383 |
BSEP_Gr | 5′-GCGATGAGCAACCGAAATGA | 1955-1974 |
BSEP_Hf | 5′-GATGGGATTCTTTACTGGATTC | 1089-1110 |
BSEP_Hr | 5′-CCTTCACTGGGGTCATAGAA | 1537-1556 |
BSEP_If | 5′-GCAGCTCGTCAGATACAGAA | 625-644 |
BSEP_Ir | 5′-CAGAAGGCCAATGCATAACA | 1132-1151 |
BSEP_Jf | 5′-TGAAGGCCTATGCCAAAGC | 938-956 |
BSEP_Jr | 5′-TTCACCCTTAATTCGATCCA | 1364-1383 |
BSEP_Kf | 5′-ATTGTTTCGATTTTCTTCA | 273-291 |
BSEP_Kr | 5′-GCTTTGGCATAGGCCTTCA | 938-956 |
BSEP_Lf | 5′-AACCCTTGTCCAGATTTTCCTC | 1197-1218 |
BSEP_Lr | 5′-ATGATGGGTTTCCGGTCTATTG | 1313-1334 |
FullBsep_felinef | 5′-TTGCAATTACCATGTCTGACTCAGTAATTCTTCGC | |
FullBsep_feliner | 5′-TCAACTGATGGGGGCTCCTGTGATGACTAG | |
FullBsep_caninef | 5′-TTGCAATTACCATGTCTGATGCAGTAATTCTTCGC | |
FullBsep_caniner | 5′-TCAACTGATGGGGGCTCCTGTGGTAACTAG | |
FullBSEP + AttB1_humanf | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCCA CCATGTCTGACTCAGTAATTCTTC | |
FullBSEP + AttB1_humanr | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAAC TGATGGGGGATCCAGTG | |
AttB1_felinef | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCC ACCTTGCAATTACCATGTCTGACTCAGTAATTCTTCGC | |
AttB1_feliner | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAAC TGATGGGGGCTCCTGTGATGACTAG | |
AttB1_caninef | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCC ACCTTGCAATTACCATGTCTGATGCAGTAATTCTTCGC | |
AttB1_caniner | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTCAAC TGATGGGGGCTCCTGTGGTAACTAG |