Table 1 Primer sequences used in real-time PCR analysis and ChIP assay
Target gene | Sequence (F: forward, R: reverse) | GenBank access | Applications |
|---|---|---|---|
IL-1β | F: 5′-AACATCGCCACCTACAAG-3′ | Y15006 | mRNA quantification |
R: 5′-TACTCGGTACATACGAGATGGAAA-3′ | |||
IL-6 | F: 5′- CATGGACTGGAGCACAAGTA-3′ | AJ309541.1 | mRNA quantification |
R: 5′-TGGAGAGCAGCCCATGTAA-3′ | |||
FTO | F: 5′-TGAAGGTAGCGTGGGACATAGA-3′ | NM_001185147 | mRNA quantification |
R: 5′-GGTGAAAAGCCAGCCAGAAC-3′ | |||
TLR2 | F:5′-ATCCTGCTGGAGCCCATTCAGAG-3′ | NM_ 204278.1 | mRNA quantification |
R:5′-TTGCTCTTCATCAGGAGGCCACTC -3′ | |||
TLR4 | F: 5′-GTTTGACATTGCTCGGTCCT-3′ | NM_001030693 | mRNA quantification |
R: 5′-GCTGCCTCCAGAAGATATGC-3′ | |||
C/EBPβ | F: 5′- 5′-ACGAGGCGGACTGTTTGG-3′ | NM_205253 | mRNA quantification |
R: 5′- GCTGCTGGGATGCTGCTAA-3′ | |||
STAT3 | F: 5′- CAACAACCCCAAGAAC-3′ | NM_001030931.1 | mRNA quantification |
R: 5′- GCTGAGACCACGCTTT-3′ | |||
β-Actin | F: 5′-TGCGTGACATCAAGGAGAAG-3′ | NM_205518 | mRNA quantification |
R: 5′-TGCCAGGGTACATTGTGGTA-3′ | |||
Fragment 1 | F: 5′- AGTTTGCCTCATGCTGGTA-3′ | ENSGALG00000003591 | ChIP |
R: 5′- CTGTAGCGAAGTTGGGTGG-3′ | |||
Fragment 2 | F: 5′- CCAGGAGTTCCAGCATTA-3′ | ENSGALG00000003591 | ChIP |
|  | R: 5′- ATGGCTCCATCCAGTTGC-3′ |  |  |