Table 1 Characteristics of VNTR loci used in MLVA for F. noatunensis subsp. noatunensis isolates
Marker locus | Alternate designationa | GeneBank accession no. | Repeat sequence | Number of repeats | Fragment size range (bp) | No. of alleles | DI (%) | Coding functions |
|---|---|---|---|---|---|---|---|---|
fnVNTR-1 | NA | CP000937 | TCTTTATTG | 20-33 | 427-549 | 9 | 76.5 | TPR repeat protein |
fnVNTR-2 | Fnn-VNTR2 | GU385768 | AGTTATT | 8-14 | 275-440 | 4 | 57.7 | ThiJ/PfpI family protein |
fnVNTR-3 | Fnn-VNTR3 | GU385769 | TAGAT | 7-11 | 213-234 | 5 | 67.6 | UDP-N-acetylglucosamine 2-epimerase |
fnVNTR-4 | Fnn-VNTR1 | GU385767 | TTAAGGTA | 4-7 | 507-532 | 5 | 65.5 | NA |
fnVNTR-5 | Fnn-VNTR4 | GU385770 | CCACAA | 9-31 | 404-539 | 7 | 65.5 | DNA-directed DNA polymerase |