Figure 5
Effect of serotonin and ritanserin on cell viability is cell line dependent. a: Effects of serotonin and ritanserin on cell viability in CnOb. Cell viability was assessed through a MTS assay of CnOb treated with escalating doses of serotonin or ritanserin. Decreased cell viability relative to the vehicle control is only observed at 50 μM serotonin (p = 0.013) and 50 μM ritanserin (p < 0.01). A greater decrease in viability is observed at 50 μM ritanserin than 50 μM serotonin (p < 0.01). Plotted values are mean cell viability +/− 95% confidence interval of mean. b: Effects of serotonin and ritanserin on cell viability in COS. Cell viability was assessed through a MTS assay of COS treated with escalating doses of serotonin, ritanserin, and ritanserin with concurrent serotonin (12.5 μM). Relative to the vehicle control, increased viability was observed at 3.125, 6.25, and 12.5 μM serotonin (p < 0.01). Conversely, a mild decrease in viability was observed at 6.25 μM ritanserin (p < 0.01) with marked decreased evident at 12.5, 25.0, and 50 μM (p < 0.01). Viability was greater at 6.25 and 12.5 μM ritanserin when concurrently incubated with serotonin (12.5 μM) than the viability observed with 6.25 and 12.5 μM ritanserin alone (p < 0.01). The calculated IC50 of ritanserin for COS is 6.3 μM. Plotted values are mean cell viability +/− 95% confidence interval of mean.